LOCUS       40924_16385        4436 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Phage display vector pdvec0 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Makino Y, Ishida S, Itoh N.
  TITLE     Phage display vector pdvec0
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Makino Y, Itoh N.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2018) Contact:Yoshihide Makino Toyama Prefectural 
            Univerisity, Department of Biotechnology, Faculty of Engineering; 
            5180 Kurokawa, Imizu-city, Toyama 939-0398, Japan URL 
            :http://www.pu-toyama.ac.jp/
REFERENCE   3  (bases 1 to 4436)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4436)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JUL-2018) Contact:Yoshihide Makino Toyama Prefectural 
            Univerisity, Department of Biotechnology, Faculty of Engineering"; 
            volume: " 5180 Kurokawa, Imizu-city, Toyama 939-0398, Japan URL 
            :http"; pages: "//www.pu-toyama.ac.jp"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    217..270
                     /gene="III"
                     /note="gene III signal peptide of Mq3KO7. This region
                     introduces the express of a protein structural gene 
                     inserted in the downstream of this region."
     misc_feature    271..336
                     /gene="III"
                     /note="A protein structural gene can be inserted in this
                     region. The inserted gene is expressed as a fusion peptide 
                     of (geneIII signal peptide of M13KO7)-(polypeptide from 
                     inserted sequence)-(geneIII mature peptide M13KO7)."
     CDS             334..1554
                     /label=M13 gene III
                     /note="pIII"
     primer_bind     complement(1565..1581)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1794..2249
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2531..2635
                     /label=AmpR promoter
     CDS             2636..3493
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3667..4255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga aaaaattatt attcgcaatt
      241 cctttagttg ttcctttcta ttctcactcg gccggctcgg cccatatgta atgatagtaa
      301 tgatagctcg aggaattcgg ggcctcaggg gcctctgcag aaactgttga aagttgttta
      361 gcaaaacccc atacagaaaa ttcatttact aacgtctgga aagacgacaa aactttagat
      421 cgttacgcta actatgaggg ctgtctgtgg aatgctacag gcgttgtagt ttgtactggt
      481 gacgaaactc agtgttacgg tacatgggtt cctattgggc ttgctatccc tgaaaatgag
      541 ggtggtggct ctgagggtgg cggttctgag ggtggcggtt ctgagggtgg cggtactaaa
      601 cctcctgagt acggtgatac acctattccg ggctatactt atatcaaccc tctcgacggc
      661 acttatccgc ctggtactga gcaaaacccc gctaatccta atccttctct tgaggagtct
      721 cagcctctta atactttcat gtttcagaat aataggttcc gaaataggca gggggcatta
      781 actgtttata cgggcactgt tactcaaggc actgaccccg ttaaaactta ttaccagtac
      841 actcctgtat catcaaaagc catgtatgac gcttactgga acggtaaatt cagagactgc
      901 gctttccatt ctggctttaa tgaggatcca ttcgtttgtg aatatcaagg ccaatcgtct
      961 gacctgcctc aacctcctgt caatgctggc ggcggctctg gtggtggttc tggtggcggc
     1021 tctgagggtg gtggctctga gggtggcggt tctgagggtg gcggctctga gggtggcggt
     1081 tccggtggtg gctctggttc cggtgatttt gattatgaaa agatggcaaa cgctaataag
     1141 ggggctatga ccgaaaatgc cgatgaaaac gcgctacagt ctgacgctaa aggcaaactt
     1201 gattctgtcg ctactgatta cggtgctgct atcgatggtt tcattggtga cgtttccggc
     1261 attgctaatg gtaatggtgc tactggtgat tttgctggct ctaattccca aatggctcaa
     1321 gtcggtgacg gtgataattc acctttaatg aataatttcc gtcaatattt accttccctc
     1381 cctcaatcgg ttgaatgtcg cccttttgtc tttagcgctg gtaaaccata tgaattttct
     1441 attgattgtg acaaaataaa cttattccgt ggtgtctttg cgtttctttt atatgttgcc
     1501 acctttatgt atgtattttc tacgtttgct aacatactgc gtaataagga gtcttaagct
     1561 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     1621 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     1681 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     1741 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     1801 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1861 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1921 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     1981 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     2041 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2101 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     2161 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2221 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     2281 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2341 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2401 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     2461 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     2521 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2581 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2641 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2701 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     2761 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2821 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2881 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2941 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3001 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3061 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3121 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3181 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3241 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3301 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3361 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3421 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3481 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3541 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3601 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3661 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3721 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3781 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3841 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3901 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3961 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4021 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4081 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4141 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4201 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4261 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4321 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4381 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
